›› 2012, Vol. 24 ›› Issue (3): 227-230.doi: 10.3969/j.issn.1004-616x.2012.03.014

• 肿瘤防治 • 上一篇    下一篇

维吾尔族妇女宫颈癌发生与EBV及HPV感染的关系

阿比班·阿克拉,米克热依·玉山江,玛依努尔·尼牙孜,阿布力孜·阿布杜拉,阿比达·阿布都卡德尔   

  1. 1. 新疆医科大学生物化学暨分子生物学教研室，新疆 乌鲁木齐 830011；2. 新疆维吾尔医医院麻醉科，新疆 乌鲁木齐 830049；3. 新疆维吾尔自治区人民医院妇科，新疆 乌鲁木齐 830001；4. 新疆医科大学第一附属医院妇科，新疆 乌鲁木齐 830054
  • 收稿日期:2011-12-05 修回日期:2012-01-13 出版日期:2012-05-30 发布日期:2012-05-30
  • 通讯作者: 阿比达·阿布都卡德尔

The role of EBV and HPV infection in cervical cancer development of Uyghur women

Habiba·Akiram，Mikereyi·Yushanjiang，Mayinuer·Niyazi ，Abulizi·Abudula，Abida·Abudoukadeer   

  1. 1. Department of Biochemistry and Molecular Biology, Xinjiang Medical University, Urumqi 830011; 2. Xinjiang Hospital of Uyghur Medicine, Urumqi 830049; 3. General Hospital of Xinjiang
  • Received:2011-12-05 Revised:2012-01-13 Online:2012-05-30 Published:2012-05-30
  • Contact: Abida·Abudoukadeer

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摘要： 目的： 探讨维吾尔族妇女宫颈癌发生过程中EB病毒(Ebstein-Barr virus，EBV)及人乳头瘤病毒(human papillomavirus， HPV)感染的作用及其意义。方法：收集维吾尔族妇女宫颈炎、宫颈内上皮瘤样病变(cervical intraepithelial neoplasia，CIN) Ⅰ/Ⅱ/Ⅲ和宫颈鳞癌患者福尔马林浸泡与石蜡包埋组织标本共178例，提取DNA并采用PCR方法对EBV和HPV DNA进行检测；用免疫组织化学方法检测宫颈癌EBV蛋白表达。结果：病毒DNA检测结果显示，宫颈炎、CINⅠ、CINⅡ~Ⅲ和宫颈癌患者各组HPV DNA检出率依次是2.5%、12.5%、68.0%、96.4%，EBV DNA为0、3.1%、28.0%和69.6%，其中宫颈炎组与CINII~III和宫颈癌组间的差异具有统计学意义(P均<0.05)，但宫颈炎与CINI组间的差异无统计学意义(P>0.05)。宫颈病变病理进程不仅伴随着 HPV及EBV DNA阳性检出率梯度上升，而且与HPV和EBV双重感染检出率增高呈正相关(r =0.46， χ 2=82.50，P＜0.01)。对宫颈癌组织进行免疫组织化学分析，发现EBV DNA检测阳性标本中EBV蛋白阳性表达检出率为89.7%(34/39)，而阴性标本中蛋白表达阳性率为6%(1/17)。结论：维吾尔族妇女宫颈癌的发生与发展可能与HPV和EBV双重感染密切相关。

关键词: 宫颈癌 , 人乳头瘤病毒, EB病毒

Abstract: OBJECTIVE: Study on the role of Ebstein-Barr virus (EBV) and human papillomavirus (HPV) infection in the development of cervical cancer of Uyghur women. METHODS：We collected 178 cases of formalin-fixed and paraffin-embedded cervical tissue specimens of Uyghur women with cervicitis，cervical intraepithelial neoplasia (CIN)Ⅰ/Ⅱ/Ⅲ and cervical squamous cell carcinoma (CSCC). EBV and HPV DNA was detected by polymerase-chain reaction (PCR) with the tissue DNA extracted，and the EBV protein expression was checked by immunohistochemistry. RESULTS：HPV DNA was detectable in 2.5%，12.5%，68.0% and 96.4% cases of cervicitis，CINⅠ，CINⅡ-Ⅲ and cervical cancer，respectively；and EBV DNA in 0%，3.1%，28.0% and 69.6% cases， respectivelly；with a significant difference among groups of cervicits ，CINII-III and cancer for both HPV and EBV(P>0.05). Further analysis indicated that cervical lesion pathogenesis was not only accompanied with gradual increasing rate for HPV or EBV DNA alone，but also positively correlated with the increase of HPV-EBV dual-infection(correlation coefficient r=0.46；χ2=82.50， P＜0.01). EBV protein expression was 89.7% positive in EBV-DNA positive cases(34/39)and 6% positive in EBV-DNA negative cases (1/17). CONCLUSION：Uyghur cervical cancer development and progression may be closely associated with the dual infection by HPV and EBV.

Key words: cervical cancer, human papillomavirus, Ebstein-Barr virus

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